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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Published by Universitas Gadjah Mada
ISSN : 01261312     EISSN : 23563931     DOI : http://dx.doi.org/10.19106/JMedSci005303202101
Core Subject : Science,
Immunology & microbiology Neuroscience
Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality articles in the areas of biomedical science from basic to clinical sciences.The journal welcomes papers from original articles, case reports, reviews, and book reviews. All papers published in JMedSci are freely available as downloadable pdf files. The journal began its publication on March 1973 and published quarterly (January, April, July, and October). JMedSci is abstracted and indexed in DOAJ, Crossref, Google Scholar, Sinta, Indonesia One Search. JMedSci is accredited by Directorate of General Higher Education, the Ministry of Research, Technology, and Higher Education, Indonesia
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 18 Documents
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Search results for , issue "Vol 41, No 04 (2009)" : 18 Documents clear
The effect of metformin on proliferation and glucose uptake in keloid fibroblast culture Yohanes Widodo Wirohadidjojo, Nur Dwita Larasati, Sunardi Radiono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 04 (2009)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Metformin as an antihyperglycemic agent has a potential effect in increasing type I collagen synthesis and decreasing MMP, so that it has a potential to be an antiaging agent. One of aging failure processes is the development of keloids. Keloids are formed due to hyperproliferation of fibroblasts, an increase of collagen synthesis, particularly type I and III, and a decrease in MMP-1 and MMP-2. Fibroblast proliferation process and collagen synthesis need glucose uptake. The study on metformin ability to aggravate or stimulate the formation of keloid has never been conducted before. Objective: The aim of this study was to know the difference of proliferation and glucose uptake between keloid fibroblasts given metformin and without metformin. Method: A simple experiment was conducted using 3rdpassage keloid fibroblasts culture. Keloid fibroblasts were divided into 2 groups, the first group was treated with metformin in the dose of 100 pg/mL, 200 pg/mL, 300 pg/ mL, 400 pg/mL, and control. Keloid fibroblasts proliferation in the first group was measured using spectrophotometer with MTT assay, and glucose uptake of keloid fibroblast in the other group was measured using glucometer. The difference in proliferation and glucose uptake of keloid fibroblast was analyzed using one-way anova. Result: The result of this study showed that the average keloid fibroblast proliferation in the metformin treatment groups was not increased compared to that in control group. Meanwhile, the average keloid fibroblast glucose consumption in metformin treatment group significantlyincreased, at the dose of 300 ig/mL (p =0.044) and 400 I!g/mL (p = 0.0081. Conclusion: Metformin could not increase keloid fibroblasts proliferation, but it could increase glucose uptake of keloid fibroblasts.
Serum iron level after ingestion of repeated dose of iron shortly after and 2 hours after meal Sri Kadarsih Soejono, Lukman Hakim, Erna Kristin, Mohammad Hakim,
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 04 (2009)
Publisher : Universitas Gadjah Mada

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Abstract

Background: The prevalence of iron deficiency anemia in pregnant women is estimated to be higher than nonpregnant women. Ironsupplementation program has been proven clinicallyto improvethe hemoglobin (Hb)level of pregnant women. The absorption of iron is affected by food, therefore iron has to be given 2 hours after meal. However, in practice, iron was given shortly after meal. The physiologicalchange in pregnant women affects drug absorption, distribution, and eliminationphases. Objective: To understand serum iron level after ingestion of repeated dose of iron shortly after and 2 hours after meal for 12 weeks in trimester 2 pregnant women with iron deficiency anemia. Method: The research design was a phase IIclinical trial. Subjects were 24 trimester 2 pregnant women with iron deficiency anemia, classified into two groups, who were treated as follows: The first group was consisted of 12 women with irondeficiency anemia, treated with twice-a-day ferrous sulphate tablet @300 mg orally,given shortly after meal for 12 weeks; the second group was consisted of 12 women with iron deficiency anemia, treated with twice-a-day ferrous sulphate tablet @300 mg orally, given 2 hours after meal for 12 weeks. Bloodsamples were taken in week 2, 4, 6, 8, 10, and 12 after treatment. Serum (ferric) iron level was measured with Vitros Fe Slides method. Result: Minimum,maximum, and average steady-state ironlevels (Css min, Css max, Css average) of treatment 1 were 104.1 ::I:14.03 ug/dL, 96.44::1:13.22 ug/dL, and 112.38::1:14.03 ug/dL (mean ::I:SEM),respectively; while minimum, maximum, and average steady-state iron levels (Css min, Css max, Css average) of treatment 2 were 125.77::1:9.31 ug/dL, 118.03::1:9.21 ug/dL, and 125.77::1:9.31 ug/dL (mean::l:SEM),respectively. No statistical significant difference was found within treatment in minimumsteady-state level between week 2, 4, 6, 8, 10, 12 after treatment. There was also no significant difference in minimumsteady-state level between treatment groups in week 2, 4, 6, 8, 10, and 12. Conclusion: There were no differences in serum iron level after ingestion of repeated dose of ironshortly after and 2 hours after meal for 12 weeks intrimester 2 pregnant women with iron deficiency anemia.
The effect of zinc supplementation on collagen of periodontitisrat Soeharyo Hadisaputro, Suryono, Praptiwi, Siti Fatimah Muis
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 04 (2009)
Publisher : Universitas Gadjah Mada

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Abstract

Background: Zinc,cofactors of DNA-and RNA-polymerase, having great role in tissue heal-ing. In periodontitis, collagen type 1 the main fiberconstituting periodontal structure is des-troyed and be the main cause of lost of teeth among adult in Indonesia. Zndeficiency is still a nutritional problem. The aim of the study is to obtain the influence of 500 pg Zn supplementation (Zn,1and 200 pg (Zn2)per day for 7 days to periodontal collagen of Periodontitis (PI rat through histologic feature. Method: inthe experimental study with factorial design, 29 adult male Wistar rat were used. Subjects were grouped simple randomly into 6 i.e Healthy (H)at the start, Hatthe end, p, P+Te-tracycline IT), ,P + T+ Zn" and P+ T+ Zn2.Subject other than Hgroups were induced periodontitisby Porphyromonasgingivalisbacteria. Zn concentration was measured by AAS Flame, Periodontal tissue was stained with Mallory,s. T test and ANOVAwas used to analyse difference between mean of Znconcentration of groups studies. Result: There were similarity in the feature of groups H at the start, Hat the end, and P+ T+ Zn" in sense of regularity, length, and solidity of collagen. P group had irregular, and short collagen. Groups of P+ T with shorter collagen, had similarity to Health at the end. P+ T+ Zn2with shorter collagen similar to P+T+ Znl. There was significant difference inZn concentrat-ion between Hat the end and Pgroup. Nosignificant differencies among Zn concentrations of Psubject groups. Great variety of Zn concentration found among subjects of groups prob-ably were the cause of absence of difference, although means of the Zn concentration values depicted it. Conclusion: Zn supplementation dosage 500 pg/day given to periodontitis rat beside Tetra-cycline, gives better effect to collagen structure compared to 200 pg/day.
Atypical carcinoid of the lung: A case report Soeripto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 04 (2009)
Publisher : Universitas Gadjah Mada

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Abstract

A 3D-year old woman came to Dr Sardjito Hospital with chief complain of dyspneu that she had since 1 month before her visit. Clinicalexamination revealed a dull percussion below. The second intercostal of the left chest. The chest X ray revealed massive pleural effusion in the left chest, could not exclude a mass in left lung. Thoracoscopic biopsy of the left lung mass was done. Macroscopic examination of lung tissue sample revealed fragmented tissue, with volumeof 0.25 cc, blackcolour, and rubberyconsistency. The diagnosis of atypicalcarcinoid with small cell carcinoma as a differential diagnosis was determined based on morphological pattern. Immunohistochemical staining examination showed expression of cytokeratin, CAM5.2, and synaptophysin, but showed negative expression of chromogranin, CD56, and TTF-1 .A diagnosis of an atypical carcinoid was confirmed based on the immunohistochemicalexamination. A very rare case of atypical carcinoid of the lung in a non-smoker 3D-yearoldwoman with morphologicaldiagnostic problem solved by immunohistochemical staining was reported.
Human sexual dimorphism: from an evolutionary perspective to practical . overview Suriyanto, Rusyad Adi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 04 (2009)
Publisher : Universitas Gadjah Mada

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Abstract

This paper goal is to discuss human sexual dimorphism in evolutionary perspective and practical overview. Evolution of human sexual dimorphism has wide implication !n human biological study and many applications to medical field. Human sexual dimorphism has experienced changes and adaptations along its proceeding to current time and space. These changes are natural consequences. Understanding this problem as process and products of evolution, we can enter this problem into medicine and be a background. Understanding as a background, as human natural history, we will understand why human can be so now and why he! she will change, including sexual dimorphism problem. The understanding of background is needed to avoid creation of human with narrow vision, highly specialized, excessive fanatics for self competency, easily astonished, panic and immediately tricked in real life which is complex and continuously changing. Human anatomy knowledge, as one basic medical aspect, sexually presents understanding that there are changing and developing characteristics among men (males) and women (femalesl over time. The medical intentions are aspects of sexual dimorphism directly applied and related closely to medicine. The paper begins with sexual dimorphism discussion, and continued by evolutionary perspective discussion, and followed up by practical discourse. This continuity is an effort to illustrate that Homo sapiens species, like us, is always dynamic and changing, like nature which is never static neither stable.
Fibroadenoma of the breast with florid epithelial cells hyperplasia: a case report Soeripto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 04 (2009)
Publisher : Universitas Gadjah Mada

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Abstract

Fibroadenoma is a benign biphasic tumor of the breast, composed of an epithelial and a stroma component. Fibroadenoma with epithelial cells hyperplasia may contribute to the higher risk of breast cancer, especially for florid and atypical hyperplasia. The distinction between fibroadenoma with florid epithelial cells hyperplasia, in-situ carcinoma and invasive duct carcinoma of the breast ban be difficult morphologically. To solve the problem, immunohistochemical staining with specific andibodies will be helpfull in distinguishing usual duct hyperplasia from ductal carcinoma in situ. A case of fibroadenoma with florid epithelial cells hyperplasia has been reported. Morphologically, this tumor is difficult to be differentiated from ductal carcinoma. Immunohistochemical staining with High molecular weight cytokeratins 34BE12, Smooth Muscle Actin (SMA),E-cadherin could be used to determine the diagnosis of this tumor.
The effect of operating room design and behavior of operating room staff on surgical site infection (881) incidence Iwan Dwiprahasto, Yulianto Widodo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 04 (2009)
Publisher : Universitas Gadjah Mada

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Abstract

Introduction: In surgery, medical error is often related to surgical site infection (551).Santa Maria Hospital, Pemalang, is a private hospital which gives priority to quality and applies the patient safety principle. During 2006, there were 7 cases of 551 in 785 surgical cases in Santa Maria Hospital, Pemalang, but this number was considered as the tip of an iceberg. The high microorganism rate, operating room design which was not agreeable with the standard by Kepmenkes No 1204/MENKES/SK/X/2004, and the behavior of operating room staff were the risk factors of 55!. ObJective:To find out the effect of the change in operating room design and behavior of operating room staff on 551 incidence in Santa Maria, Pemalang, and to find out the risk factors of 55!. Method: It was a quasi-experimental study with pre- and post-test without control group design. Population were all operating room staff, while subject of this study was operating room design. Dependent variable was 551incidence, while independent variables were behavior of operating staff and operating room design. Data were analyzed with independent t-test, chi-square test, and multivariate logistic regression. Re.ult: The change in operating room design and operating room staff behavior has significantly decreased the incidence of 551 (OR = 3.646; 95%CI = 1.24 < OR< 10.77). Conclu.lon: Intervention on operating room design and staff behavior has decreased the incidence of 55!. Risk factors of 551were operating room design, operating room staff behavior, and duration of surgery.
The risk factor of urinarytract infection in patient with leukemia Pungky Ardani Kusuma, Melati Dini Hari, Soetaryo,
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 04 (2009)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Urinary tract infection (UTI) is one of most common infectious diseases in patients wuth leukemia. Some researchers showed that the prevalence of UTI was the third highest after pneumonia and gastrointestinal tract infection among children with leukemia. UTI may be affected by many factors, i.e. host, microorganism, and environment. E. coli, Klebsiella, dan Proteus are the most common bacterial etiology of UTI in leukemia. Objective: The aim of this research was to know the risk factor of UTI in children with leukemia. Method: This research used case-control design. Subjects of this study were children (0-15 years old) with leukemia who came to Department of Pediatrics, Dr. Sardjito Hospital since January st, 2000 until October 3", 2008. There were 38 patients in case group and 109 patients in the control group. Data collected from medical records were age, gender, nutritional status, type of leukemia, treatment phase of leukemia, and absolute neutrophil count. Risk factors analyzed were age, gender, nutritional status, and neutropenia. UTI was diagnosed by bacterial culture and bacterial count. Result: Univariate analysis showed that female gender was the risk factor of UTI (OR 2.35; CI 1.10- 5.00), meanwhile age less than 5 years old (OR 0.48; CI 0.22-1.06L poor-fair nutritional status (OR 1.20; CI 0.52-2.78), and neutropenia (OR 0.73; CI 0.35-1.55) were not risk factors of UTI. Multivariate analysis showed that female gender was the risk factor of UTI (OR 2.36; CI 1.08-5.14). Conclusion: Female gender was a risk factor of UTI in children with leukemia Keywords: leukemia - children - gender - urinary tract infection - risk factors
Electrocardiographic pattern changed in hospitalized leptospirosis and its association with disease severity Bambang Irawan, Anggoro Budi Hartopo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 04 (2009)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Electrocardiographic (ECG)changes have been observed in leptospirosis. The prevalence of ECG alterations may reach 80%. Despite of this finding,few studies have been conducted to assess ECGpatterns in only hospitalized leptospirosis patients in Indonesia. Objective: To reveal the prevalence and type of ECGalterations in hospitalizedleptospirosisand its association with disease severity. Methods: This was a cross-sectional study using medical record data. Patients hospitalized in Dr. Sardjito Hospital (January 2003 - December 2007) with leptospirosis (lCDXA27.9) were enrolled. Electrocardiogramstaken in 24 hours of admission were evaluated. Disease severity was determined based on clinical and laboratory findings. Association between disease severity with ECGrecordings were performed using Kruskal-Wallisand Chi square test. P value < 0.05 was significant. Results: Sixtysamples were analyzed.The prevalence of alterationof ECGrecordingswas 66.7%. Sinustachycardia was the most frequent (36.7 %). The most common arrhythmia was atrial fibrillation(10 %). Other abnormalities were alterationof ventricularrepolarization(8.3%1.extrasystoles (6.6%) and disorderof conduction (4.9%). Although not significant, those with atrial fibrillationtended to have the worst clinicaland laboratory findings. Conclusions: The prevalenceof alterationof ECGrecordingsin hospitalizedleptospirosiswas 66.7 %. Atrialfibrillation was the most frequent arrhythmia, and patients with this arrhythmia were likelyto have the most severe disease.
The effect of zinc supplementation on collagen of periodontitisrat Praptiwi, Siti Fatimah Muis Soeharyo Hadisaputro, Suryono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 04 (2009)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Zinc,cofactors of DNA-and RNA-polymerase, having great role in tissue heal-ing. In periodontitis, collagen type 1 the main fiberconstituting periodontal structure is des-troyed and be the main cause of lost of teeth among adult in Indonesia. Zndeficiency is still a nutritional problem. The aim of the study is to obtain the influence of 500 pg Zn supplementation (Zn,1and 200 pg (Zn2)per day for 7 days to periodontal collagen of Periodontitis (PI rat through histologic feature. Method: inthe experimental study with factorial design, 29 adult male Wistar rat were used. Subjects were grouped simple randomly into 6 i.e Healthy (H)at the start, Hatthe end, p, P+Te-tracycline IT), ,P + T+ Zn" and P+ T+ Zn2.Subject other than Hgroups were induced periodontitisby Porphyromonasgingivalisbacteria. Zn concentration was measured by AAS Flame, Periodontal tissue was stained with Mallory,s. T test and ANOVAwas used to analyse difference between mean of Znconcentration of groups studies. Result: There were similarity in the feature of groups H at the start, Hat the end, and P+ T+ Zn" in sense of regularity, length, and solidity of collagen. P group had irregular, and short collagen. Groups of P+ T with shorter collagen, had similarity to Health at the end. P+ T+ Zn2with shorter collagen similar to P+T+ Znl. There was significant difference inZn concentrat-ion between Hat the end and Pgroup. Nosignificant differencies among Zn concentrations of Psubject groups. Great variety of Zn concentration found among subjects of groups prob-ably were the cause of absence of difference, although means of the Zn concentration values depicted it. Conclusion: Zn supplementation dosage 500 pg/day given to periodontitis rat beside Tetra-cycline, gives better effect to collagen structure compared to 200 pg/day.

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